Uncaging Rub-4-AP for Neuronal Manipulation

Rub-4-AP can be uncaged with either 1 photon excitation (λ = 480 nm) or 2-photon excitation (800 nm) (Nikolenko et al., 2005 (link); Fino et al., 2009 (link)). Uncaging was performed with 470 nm LED light (Figure 1A, Thorlabs, Newton, NJ) either broadly to the entire cortex or focally, delivered through an optical fiber (OD: 200 μm, Thorlabs, Newton, NJ), using a high power LED driver (LEDD1B, Thorlabs, Newton, NJ), controlled by a stimulator (Master-8; AMPI, Jerusalem, Israel). Optical power delivered at the fiber tip was calibrated with a PM100USB power meter (Thorlabs, Newton, NJ).

Free full text: Click here

Zhao M., McGarry L.M., Ma H., Harris S., Berwick J., Yuste R, & Schwartz T.H. (2015). Optical triggered seizures using a caged 4-Aminopyridine. Frontiers in Neuroscience, 9, 25.

Publication 2015

LEDD1B PM100USB power meter

Ampi Cortex Fiber Light Optical

Corresponding Organization :

Other organizations : Cornell University, Columbia University, New York Hospital Queens, University of Sheffield, Neurological Surgery, NewYork–Presbyterian Hospital

Top 5 similar protocols

Variable analysis

independent variables

Type of uncaging excitation (1 photon at 480 nm or 2-photon at 800 nm)
Location of uncaging (broad to the entire cortex or focal, delivered through an optical fiber)

dependent variables

Uncaging of Rub-4-AP

control variables

Optical power delivered at the fiber tip (calibrated with a PM100USB power meter)
LED light source (470 nm LED light, Thorlabs, Newton, NJ)
High power LED driver (LEDD1B, Thorlabs, Newton, NJ)
Stimulator (Master-8; AMPI, Jerusalem, Israel)

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!