Thirty Wistar albino adult male rats of about 150 g body weight (b wt) were used in this work. Rats were kept in our animal facility for about 1 week before starting the experiment, for acclimatisation to the laboratory conditions.
Animals were housed in small plastic cages, maintained under constant conditions and were fed on a standard basal diet, as previously described in Khayyat et al. (2017) (link). Our experimental procedures were approved by the Menoufia University IACUC Committee for Care of Laboratory Animals (Approval No.: MNSP155).
Sunset Yellow (CAS 2783-94-0; 90% purity) and Allura Red (CAS 25956-17-6; 80% purity) were purchased from Sigma–Aldrich (Munich, Germany).
Animals were housed in small plastic cages, maintained under constant conditions and were fed on a standard basal diet, as previously described in Khayyat et al. (2017) (link). Our experimental procedures were approved by the Menoufia University IACUC Committee for Care of Laboratory Animals (Approval No.: MNSP155).
Sunset Yellow (CAS 2783-94-0; 90% purity) and Allura Red (CAS 25956-17-6; 80% purity) were purchased from Sigma–Aldrich (Munich, Germany).
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