Hantavirus Detection in Bat and Insectivore Tissues

Bats were captured with mist nets or harp traps in caves of natural roosts in Zhejiang Province in the spring of 2011, or in villages or caves in Hubei Province in the spring of 2012 (Figure 1). According to protocols described previously [47] , insectivore animals were trapped in cages using fried foods as bait in the Inner Mongolia Autonomous Region in 2006 or in Yunnan Province in the autumns of 2010 and 2011. All animals kept were alive after capture. They were initially identified by morphological examination according to the criteria for bats described by Wang [48] and for insectivores by Chen [49] , and further confirmed by sequence analysis of the mt-cyt b gene. All animals were anesthetized with ether before surgery, and all efforts were made to minimize suffering. Tissue samples of heart, liver, spleen, lung, kidney and brain were collected from bats and insectivores for detecting hantaviruses.