A selective stain, Nile blue A, fluorescent dye is used for the detection of PHB granules [17 (link)]. Dye at 0.5μg/ml of selective media was composed and sterilized. The initially screened isolate M4 was streaked on the plates and incubated at 35°C for about 96h. As a positive mark, the colonies emit orange yellow fluorescence under a UV transilluminator.
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