ATAC-seq Library Preparation from Mononuclear Cells

ATAC libraries were prepared from approximately 50 to 75 thousand of mononuclear cells using a Tagment DNA Enzyme Kit from Illumina (Cat # 20034198) by following the Omni-ATAC protocol (23 (link)). The ATAC libraries were run on 2% agarose gel and gel band corresponding to ~200–600 bp was cut to elute DNA fragments using the MinElute^® Gel Extraction Kit (Qiagen), subjected to sequencing at the Genomics Core Facility at Marshall University with Illumina Nextseq2000.

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Povroznik J.M., Akhter H., Vance J.K., Annamanedi M., Dziadowicz S.A., Wang L., Divens A.M., Hu G, & Robinson C.M. (2023). Interleukin-27-dependent transcriptome signatures during neonatal sepsis. Frontiers in Immunology, 14, 1124140.

Publication 2023

Tagment DNA Enzyme Kit MinElute® Gel Extraction Kit Nextseq2000

A dna Agarose Atac Cells Enzyme

Corresponding Organization : West Virginia University

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Variable analysis

independent variables

Tagment DNA Enzyme Kit from Illumina (Cat # 20034198)
Omni-ATAC protocol

dependent variables

ATAC library sequencing

control variables

Approximately 50 to 75 thousand of mononuclear cells
2% agarose gel
~200–600 bp DNA fragments
MinElute® Gel Extraction Kit (Qiagen)
Illumina Nextseq2000 sequencing

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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