Immunogenic Mycobacterium tuberculosis Antigens

PPD was obtained from Staten Serum Institut. Antigen 85 complex (Ag85), ESAT-6/CFP-10, alanine- and proline-rich secreted protein (Apa), groES, alpha-crystallin (also known as heat shock protein X (HspX)), and lipoarabinomannan (LAM) were obtained from BEI Resources (NR-14855, NR-49424, NR-49425, NR-49428, NR-14862, NR-14861, NR-14860, NR-14848, respectively). The 6 well-characterized immunogenic Mtb antigens were selected as those that are commercially available and include both surface and intracellular bacterial components that are known Mtb antibody targets. Influenza hemagglutinin from A/New Caledonia/20/1999 and B/Brisbane/60/2008 (ImmuneTech) was included as an internal control.

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Mthembu M., Bowman K.A., Davies L.R., Khuzwayo S., Mazibuko L., Bassett T., Ramjit D., Mhlane Z., Karim F., Alter G., Ndung'u T, & Wong E.B. (2023). Discrepancy between Mtb-specific IFN-γ and IgG responses in HIV-positive people with low CD4 counts. eBioMedicine, 90, 104504.

Publication 2023

Ag85 Alanine Alpha crystallin Antibody Antigens Bacterial Heat shock protein Hemagglutinin Influenza Intracellular Lipoarabinomannan Proline Protein Serum

Corresponding Organization :

Other organizations : University of KwaZulu-Natal, Africa Health Research Institute, Ragon Institute of MGH, MIT and Harvard, University College London, University of Alabama at Birmingham

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Variable analysis

independent variables

PPD obtained from Staten Serum Institut
Antigen 85 complex (Ag85) obtained from BEI Resources
ESAT-6/CFP-10 obtained from BEI Resources
Alanine- and proline-rich secreted protein (Apa) obtained from BEI Resources
GroES obtained from BEI Resources
Alpha-crystallin (also known as heat shock protein X (HspX)) obtained from BEI Resources
Lipoarabinomannan (LAM) obtained from BEI Resources

dependent variables

Mtb antibody targets

control variables

Influenza hemagglutinin from A/New Caledonia/20/1999 and B/Brisbane/60/2008 (ImmuneTech) included as an internal control

controls

Positive control: Influenza hemagglutinin from A/New Caledonia/20/1999 and B/Brisbane/60/2008 (ImmuneTech)
Negative control: Not explicitly mentioned

Annotations

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