Standardized Extraction and Phytochemical Analysis of Corydalis Saxicola

The CS extract used in the present study was purchased from Chengdu DeSiTe Biological Technology Co., Ltd. (Chengdu, China). The extract was prepared by the following procedure. Briefly, CS was crushed and passed through a No. 4 pharmacopeia sieve. Then, the CS was extracted three times using 80% aqueous ethanol (CS: ethanol = 1:6) at 60°C. The extract was then combined and filtered to obtain 160 L of CS filter liquor. The filtered liquor was subsequently concentrated under reduced pressure at 60°C until no ethanol was detected to obtain the resulting concentrated solution, which was continuously concentrated and dried under reduced pressure at 60°C to obtain the final CS extract. CS and seven standards (quercetin, isoquercetin, astragalin, hypericin, kaempferol, chlorogenic acid, and isorhamnetin) were analyzed by using a HPLC system (Waters 2,695, Waters Corporation, Massachusetts, United States) and Phytochemical profiles of CS extracts were determined and compared to confirm their identities (Supplementary Figure S1; Supplementary Table S1).