Blueberry Gene Expression Profiling

Nine-year-old blueberry (V. corymbosum “Duke”) plants collected from the small berry garden of the Fruit Tree Research Institute, Chinese Academy of Agricultural Sciences were used as experimental material. Experiments were conducted from March 2019 to February 2022 at the Ministry of Agriculture Key Laboratory of horticultural crop germplasm resources utilization, Institute of Fruit Tree Research, Chinese Academy of Agricultural Sciences (Xingcheng, Liaoning, China) and College of Horticulture Sciences, Shandong Agricultural University (Tai’an, Shandong, China). To ensure the consistency of the material, fruits were collected from the top of the inflorescence (first to mature). Samples were taken from plants under the same growth conditions for organ (root, stem, leaf, flower, and fruit)-specific expression. All samples were rapidly frozen in liquid nitrogen and stored at −80°C.