RIPA buffer (Solarbio, Shanghai, China) was used to lyse cells and a BCA kit (Beyotime, China) was used to quantify protein levels. β-Actin (AbMART, Shanghai, China) was used as a loading control. The primary antibody specific for Timm13 was obtained from NOVUS (USA; NBP2-13431). Anti-rabbit and anti-mouse secondary antibodies were obtained from Invitrogen (Carlsbad, USA). An Odyssey two-color infrared laser imaging system (LI-COR Biosciences, Lincoln, NE, USA) was employed to scan the blots. The quantitative analysis of grey values was performed using ImageJ software (NIH, USA).
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