Anti-mouse ELISA kit to target IFN-γ was purchased from R&D Systems (Minneapolis, MN, USA). Assays were conducted according to the manufacturer's instructions. Plates were read out in Labsystems Multiskan MCC/340 (Fisher Scientific, Suwanee, GA, USA) and data were analyzed using DSJV ELISA software (Fisher Scientific) [21 (link)-24 (link)].
Protocol detail
ELISA for IFN-γ detection
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Assays
Elisa
Ifn γ
Mouse
Corresponding Organization :
Other organizations : Greenslopes Private Hospital, University of Queensland, Thomas Jefferson University
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Variable analysis
independent variables
- Not explicitly mentioned
- IFN-γ levels
- Manufacturer's instructions were followed to conduct the assays
- Plates were read out in Labsystems Multiskan MCC/340 (Fisher Scientific, Suwanee, GA, USA)
- Data were analyzed using DSJV ELISA software (Fisher Scientific)
- Positive control: Not specified
- Negative control: Not specified
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