Micro-CT Imaging of Small Samples

MicroCT was performed using a Siemens Inveon PET/SPECT/CT Scanner (Siemens Medical Solutions, Malvern, PA, USA) as previously described (Ho et al., 2021 (link)). The cone-beam CT parameters were as follows: 360° rotation, 1080 projections, 1300-ms exposure time, 1500-ms settle time, 80-kVp voltage, 500-µA current, and effective pixel size 17.67 µm. Briefly, acquisitions were reconstructed using a Feldkamp algorithm with mouse beam-hardening correction, slight noise reduction, and 3D matrix size 1024 × 1024 × 1536, using manufacturer-provided software. Protocol-specific Hounsfield unit (HU) calibration factor was applied.

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Emmert M.E., Aggarwal P., Shay-Winkler K., Lee S.J., Goh Q, & Cornwall R. (2022). Sex-specific role of myostatin signaling in neonatal muscle growth, denervation atrophy, and neuromuscular contractures. eLife, 11, e81121.

Publication 2022

Inveon PET/SPECT/CT Scanner

Cone beam ct Ct scanner Microct Mouse Spect

Corresponding Organization :

Other organizations : University of Cincinnati Medical Center, Cincinnati Children's Hospital Medical Center, University of Connecticut, Jackson Laboratory

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Variable analysis

independent variables

Cone-beam CT parameters: 360° rotation, 1080 projections, 1300-ms exposure time, 1500-ms settle time, 80-kVp voltage, 500-µA current

dependent variables

Reconstructed 3D matrix size: 1024 × 1024 × 1536
Protocol-specific Hounsfield unit (HU) calibration factor

control variables

Siemens Inveon PET/SPECT/CT Scanner
Feldkamp algorithm with mouse beam-hardening correction and slight noise reduction

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