Genotyping Pigs for S. aureus Carrier Status

To detect QTLs associated with S. aureus carrier status, we genotyped all pigs using diagnostic blood samples collected in EDTA tubes (VWR, USA). DNA was extracted using either a salting out procedure with minor modifications [26 (link)] or MasterPure™ Complete DNA and RNA Purification Kit (Epicentre Biotechnologies, USA) according to the manufacturer’s instructions. The concentration and purity of DNA was measured on a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA). 2500 ng of each sample was submitted for genotyping to GeneSeek, Inc. (http://www.neogeneurope.com). Samples were genotyped for 61,565 SNPs using Illumina’s Porcine SNP60 beadchip [25 (link)].

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Skallerup P., Espinosa-Gongora C., Jørgensen C.B., Guardabassi L, & Fredholm M. (2015). Genome-wide association study reveals a locus for nasal carriage of Staphylococcus aureus in Danish crossbred pigs. BMC Veterinary Research, 11, 290.

Publication 2015

EDTA tubes MasterPure™ Complete DNA and RNA Purification Kit NanoDrop 1000 spectrophotometer Porcine SNP60 beadchip

Blood Diagnostic Edta Porcine Qtls Snps

Corresponding Organization :

Other organizations : University of Copenhagen

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Variable analysis

independent variables

Genotyping all pigs using diagnostic blood samples collected in EDTA tubes (VWR, USA)

dependent variables

S. aureus carrier status

control variables

DNA extraction using either a salting out procedure with minor modifications [26 (link)] or MasterPure™ Complete DNA and RNA Purification Kit (Epicentre Biotechnologies, USA) according to the manufacturer's instructions
Concentration and purity of DNA measured on a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA)
2500 ng of each sample submitted for genotyping to GeneSeek, Inc. (http://www.neogeneurope.com)

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