Histomorphometric Analysis of Vascular Tissue

The harvested vessels were formalin-fixed and processed for standard paraffin embedding for histomorphometry. The deparaffinized sections of 5μm thickness were used for H&E and trichrome staining for assessing the vascular morphology and ECM organization following our previous protocols [19 (link), 20 (link)]. The stained slides were mounted with xylene-based mounting media and imaged using a slide scanner microscope attached with an imaging camera (Leica Thunder, Germany).

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Thankam F.G., Rai T., Liu J., Tam J, & Agrawal D.K. (2022). Minimally Oxidized-LDL-Driven Alterations in the Level of Pathological Mediators and Biological Processes in Carotid Atherosclerosis. Cardiology and cardiovascular medicine, 6(2), 137-156.

Publication 2022

slide scanner microscope imaging camera

Formalin Microscope Vascular Xylene

Corresponding Organization :

Other organizations : Western University of Health Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Vascular morphology
ECM organization

control variables

Tissue preparation (formalin-fixed, paraffin-embedded)
Staining protocols (H&E, trichrome)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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