Protein Identification via Mass Spectrometry

Protein bands were stained using the ProteoSilver Plus Silver Stain Kit (Sigma, St. Louis, MO) according to the manufacturer’s protocol and excised from the polyacrylamide gel. After washing, the gels were digested by incubation in buffer with trypsin [32] (link). The tryptic digests were analyzed by an LC-MS/MS system as described previously [33] (link). Database search was performed using MASCOT software (version 2.2.1., Matrix Science Ltd., London) and the NCBI Refseq sequence database under the parameters as described previously [34] (link).

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Tanabe K., Yamazaki H., Inaguma Y., Asada A., Kimura T., Takahashi J., Taoka M., Ohshima T., Furuichi T., Isobe T., Nagata K.I., Shirao T, & Hisanaga S.I. (2014). Phosphorylation of Drebrin by Cyclin-Dependent Kinase 5 and Its Role in Neuronal Migration. PLoS ONE, 9(3), e92291.

Publication 2014

ProteoSilver Plus Silver Stain Kit MASCOT software

Buffer Gels Ms ms Polyacrylamide gel Protein Silver Stain Tryptic

Corresponding Organization :

Other organizations : Tokyo Metropolitan University, Gunma University, Aichi Developmental Disability Center, Waseda University, Tokyo University of Science

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Variable analysis

independent variables

Protein bands were stained using the ProteoSilver Plus Silver Stain Kit (Sigma, St. Louis, MO) according to the manufacturer's protocol

dependent variables

The tryptic digests were analyzed by an LC-MS/MS system

control variables

Protein bands were excised from the polyacrylamide gel
The gels were digested by incubation in buffer with trypsin
Database search was performed using MASCOT software (version 2.2.1., Matrix Science Ltd., London) and the NCBI Refseq sequence database under the parameters as described previously [34]

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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