Genetic engineering of S. pombe strains

Cells were cultured on yeast extract (YE), and on yeast nitrogen base glutamate (YNG) agar plates containing the required supplements (concentration 250 mg/l on YE, 75 mg/l on YNG). Crosses were performed on malt extract (ME) agar containing supplements at a final concentration of 50 mg/l (Sabatinos and Forsburg 2010 (link)).
All Schizosaccharomyces pombe strains used for this study were either published previously, or have been generated from existing strains by crossing (see Table S1). Different ade6 alleles (Table S2) were introduced by crossing the respective mutant ade6 strain with ade6⁺ strains carrying the ura4⁺ and his3⁺ artificially introduced markers (aim) (UoA95, UoA96, UoA97, UoA98) (Osman et al. 2003 (link)). The point mutations in the ade6 alleles were verified by Sanger DNA sequencing (Source BioScience, Nottingham, UK) (Table S2).
Epitope tagging of hop1⁺ with a C-terminal 13myc-kanMX6 tag has been described in detail (Brown et al. 2018 (link)).