IOP of mice was measured using an induction-impact tonometer (Tonolab tonometer, Colonial Medical Supply, Franconia, NH) for rodents according to the manufacturer’s recommended procedures. The IOP of rabbits was measured using a Tono-Pen AVIA (Reichert Technologies, Depew, NY) while rabbits were held in a rabbit restrainer. Six consecutive IOP readings were averaged. For each mouse, the tested ophthalmic formulation (10 μl) was applied to the right eye, while the left eye received the blank and serve as a control29 (link), 60 (link). One hundred microliters of 0.9% pregabalin eye drops were applied into the inferior conjunctival sac of the right eye of the Dutch belted rabbits, while the left eye received the blank eye drops61 (link). All results were expressed as the mean percentage reduction in IOP from baseline (mean% reduction ± SEM).
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