Immunofluorescent Microscopy of Axoneme Proteins
Corresponding Organization :
Other organizations : Institut Necker Enfants Malades, Inserm, Délégation Paris 5, Sorbonne Paris Cité, Université Paris Cité, Délégation Paris 7
Variable analysis
- Fixation method (4% paraformaldehyde (PFA) for 20 min or cold methanol for 5 min at -20°C)
- Detection of axoneme proteins
- Number of ciliated cells
- Length of cilia
- Incubation in blocking buffer (10% FCS in PBS) for 30 min
- Incubation with primary antibodies diluted in blocking buffer supplemented with 0.05% saponin for 1 h at room temperature or overnight at 4°C
- Incubation with fluorescent Alexa-Fluor secondary antibodies for 1 h
- Addition of 150 μl of DAPI-Fluoromount into the Luer chamber
- None specified
- None specified
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!