Protocol detail

Quantitative Imaging of Oxidized LDL Uptake

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The U937 cells were treated with 10 μg/mL Dil-ox-LDL in RPMI 1640 medium including 10% FCS at 37 °C in 5% CO₂ for 18 h [25 (link),32 (link),36 (link)]. After twice washing with PBS gently, immunofluorescence was observed using Keyence BZ-X710 microscope and analyzed with the Keyence BZ-X710 software (Osaka, Japan). The quantification of fluorescent intensity of red color per cells was calculated as described previously [25 (link),32 (link),36 (link)].

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Terasaki M., Yashima H., Mori Y., Saito T., Shiraga Y., Kawakami R., Ohara M., Fukui T., Hirano T., Yamada Y., Seino Y, & Yamagishi S.I. (2021). Glucose-Dependent Insulinotropic Polypeptide Suppresses Foam Cell Formation of Macrophages through Inhibition of the Cyclin-Dependent Kinase 5-CD36 Pathway. Biomedicines, 9(7), 832.

Publication 2021

BZ-X710 microscope BZ-X710 software

Cells Immunofluorescence Microscope Ox ldl U937 cells

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Treatment with 10 μg/mL Dil-ox-LDL

dependent variables

Fluorescent intensity of red color per cells

control variables

RPMI 1640 medium including 10% FCS
Incubation at 37 °C in 5% CO2 for 18 h
Washing with PBS

controls

No positive or negative controls were explicitly mentioned.

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