Preparation and Use of Dopamine Uptake Inhibitors

DHβE and lidocaine were obtained from Tocris Biosciences or Ascent Scientific. Cocaine hydrochloride and nomifensine maleate were obtained from Sigma. Methylphenidate (Ritalin™) was obtained from Novartis. All drugs were dissolved in either de-ionised water, aqueous acid (nomifensine maleate) or ethanol (lidocaine) to make stock aliquots at 1000–10,000x final concentration and stored at −20 °C prior to use. DA uptake inhibitors were used at standard concentrations that have detectable outcomes on uptake kinetics concentrations but are lower than those that lead to run-down of release and/or have non-selective effects as local anaesthetics or at nAChRs^{34 (link),72 (link),73} .