Characterization of E. faecalis Biofilm Mutants

E. faecalis 12030 is a clinical strain originally isolated in Cleveland, OH [16] (link). It is a strong biofilm producer and is opsonized by antibodies against lipoteichoic acid (LTA) [16] (link). Two non-polar deletion mutants, E. faecalis 12030ΔbgsB and E. faecalis 12030ΔbgsA together with their reconstituted strains E. faecalis 12030ΔbgsB rec and E. faecalis 12030ΔbgsA rec, have been constructed and characterized previously [13] (link), [14] (link). All bacterial strains were grown at 37°C without agitation in Caso Bouillon (Carl Roth). All reagents used in this study were obtained from Sigma Aldrich if not stated otherwise.

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Diederich A.K., Wobser D., Spiess M., Sava I.G., Huebner J, & Sakιnç T. (2014). Role of Glycolipids in the Pathogenesis of Enterococcus faecalis Urinary Tract Infection. PLoS ONE, 9(5), e96295.

Publication 2014

Caso Bouillon

Antibodies Bacterial Biofilm Deletion Lipoteichoic acid Strains

Corresponding Organization : Ludwig-Maximilians-Universität München

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Variable analysis

independent variables

Two non-polar deletion mutants, E. faecalis 12030Δ bgsB and E. faecalis 12030Δ bgsA together with their reconstituted strains E. faecalis 12030Δ bgsB rec and E. faecalis 12030Δ bgsA rec

dependent variables

Biofilm production
Opsonization by antibodies against lipoteichoic acid (LTA)

control variables

Growth conditions: 37°C without agitation in Caso Bouillon (Carl Roth)
All reagents used in this study were obtained from Sigma Aldrich if not stated otherwise

controls

Positive control: None specified
Negative control: None specified

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