Chromatin Immunoprecipitation (ChIP) Analysis

Chromatin immunoprecipitation analysis was performed as previously described^{54 (link)}. Briefly, dentate gyri from mice (E0, E1, E4) were microdissected on ice and subjected to ChIP using anti-cFos antibody (Santa Cruz) following manufacturer’s instruction. The following sequence primers for qPCR were used in PCR to detect the presence of specific DNA binding to cFos: Nrxn3-Forward: 5′-GGCCTTTGTGGAGAATGAGA-3′; Nrxn3-Reverse: 5′-TTGTGGCTGGCTCTGTATTG-3′; Gria2-Forward: 5′-GGTTTGTCCCAGGCTGACTA-3′; Gria2-Reverse: 5′-GCAGCCTTCCTTTCTCTGAC-3′; Kcnj6-Forward: 5′-ATTCAGCGTCTTTGGGTTTG-3′; Kcnj6-Reverse: 5′-GCCTTAGGCTGTGTCAGAGG-3′; Kcnv2-Forward: 5′-TTTGAACCATAGCACCGACA-3′; Kcnv2-Reverse: 5′-GACTGCGAGGGTGTTTTCTC-3′; Hivep3-Forward: 5′-CCCCCTCTCTTGAGTCAGTG-3′; Kcnv2-Reverse: 5′-GTCTGGCAGAAAAGGCTGTC-3′; Nr3c1-Forward: 5′-CTGCACGGGATCTCATTTTTT-3′; Nr3c1-Reverse: 5′-GGAGTAAATGGGCAGATGGA-3′.