Three‐dimensional (3D) MRI, a method that has been proven to have strong agreement with open cadaveric dissection (considered the gold standard), and can be used interchangeably.
26 ,
27 (link) All enrolled subjects were scanned by a 3.0‐T MRI system with fully extended knees. Proton density 3D fast spin‐echo volume sequences (Skyra; Siemens, Guangzhou, China) were applied to collect Chinese images (slice thickness: 0.50 mm, voxel size: 0.3 × 0.3 × 0.5 mm) (Figure 1A). A 3.0‐T MR Scanner (Achieva; Philips, Zurich, Switzerland) with proton density turbo spin‐echo SPectral Attenuated Inversion Recovery was applied to collect Caucasian images (slice thickness: 1.00 mm, voxel size 0.12 by 2.74 by 0.12 mm). 3D models of femur and tibia were reconstructed using Amira 6.5 FEI SVG (Thermo Fisher, Stoney Creek, California, USA) according to proven and publicly available methods.
28 (link),
29 (link) With the same software, the ACL footprint areas of the femur and tibia were manually depicted on MRI images (Figure 1A).
30 (link),
31 (link) The subsequent analyses of the 3D models was completed by MATLAB2014.
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