Modulation of Peroxisome PPAR-α Pathway

The peroxisome PPAR-α agonist bezafibrate (Cayman Chemical, MI, USA), the PPAR-α antagonist GW6471 (Cayman Chemical, MI, USA), and/or the CPT1 inhibitor perhexiline maleate (Cayman Chemical, MI, USA) were cultured with Jurkat cell in vitro at the indicated concentrations^{41 (link), 42 (link)}. Catalase (Sigma-Aldrich) was used to inhibit ROS production at 1000 U/mL^{8 (link)}. perhexiline maleate was injected intraperitoneal (i.p.) to inducible liver-specific MYC oncogene transgenic mice (MYC-ON) at 8 mg/kg in 100 µL 50/50 solution of PBS/DMSO three times per week for a total of 5 weeks^{27 (link)}.

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Brown Z.J., Fu Q., Ma C., Kruhlak M., Zhang H., Luo J., Heinrich B., Yu S.J., Zhang Q., Wilson A., Shi Z.D., Swenson R, & Greten T.F. (2018). Carnitine palmitoyltransferase gene upregulation by linoleic acid induces CD4+ T cell apoptosis promoting HCC development. Cell Death & Disease, 9(6), 620.

Publication 2018

bezafibrate GW6471 perhexiline maleate Catalase

Bezafibrate Catalase Cayman Cultured cell Dmso Gw6471 Inhibit Liver Myc oncogene Perhexiline maleate Peroxisome Ppar Transgenic mice

Corresponding Organization :

Other organizations : National Institutes of Health, Center for Cancer Research, National Cancer Institute, Seoul National University, National Heart Lung and Blood Institute

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Variable analysis

independent variables

Bezafibrate (Cayman Chemical, MI, USA) at the indicated concentrations
GW6471 (Cayman Chemical, MI, USA) at the indicated concentrations
Perhexiline maleate (Cayman Chemical, MI, USA) at the indicated concentrations
Perhexiline maleate injected intraperitoneal (i.p.) to inducible liver-specific MYC oncogene transgenic mice (MYC-ON) at 8 mg/kg in 100 µL 50/50 solution of PBS/DMSO three times per week for a total of 5 weeks

dependent variables

Cell culture of Jurkat cells in vitro
ROS production

control variables

Catalase (Sigma-Aldrich) used to inhibit ROS production at 1000 U/mL

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