Adipocyte Differentiation with LPS, QCT, and SIRT-1 Inhibitor

The study employed 3T3‐L1 cell lines to examine the impact of LPS, QCT, and the SIRT‐1 inhibitor (EX‐527) on adipocytes. 1% penicillin‐streptomycin and 10% fetal bovine serum (FBS) were added to Dulbecco's Modified Eagle's Medium F‐12 (DMEM.F12) (Biosera), which was used for growing 3T3‐L1 pre‐adipocyte cells at 37°C with 5% CO². Pre‐adipocytes were differentiated 48 h after achieving 70% confluency by adding 10 μg/ml insulin, 1 μg dexamethasone, and 0.5 mM IBMX to DMEM‐F12 (differentiation media). The cells were incubated with 1 μg/mL insulin in DMEM‐F12 after 48 h of differentiation. Media was changed to differentiation one (10% FBS/DMEM‐F12) 2 days later. For 15 days, the media was replaced every 2 days.
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