The samples of CC were collected for immunofluorescence staining. The frozen sections were incubated with a primary antibody against anti-α-SMA (1:200; Affinity). Cy3-labeled goat anti-mouse IgG was used as secondary antibodies (1:500; Beyotime). Masson’s trichrome staining was performed to quantify the ratio of smooth muscle and collagen in the CC [18 (link)].
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Immunofluorescence Staining for α-SMA and Masson's Trichrome Analysis of Corpus Cavernosum
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Corresponding Organization :
Other organizations : Sun Yat-sen University, Third Affiliated Hospital of Sun Yat-sen University
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Variable analysis
independent variables
- Primary antibody against anti-α-SMA (1:200; Affinity)
- Ratio of smooth muscle and collagen in the CC
- Frozen sections
- Positive control: Not specified
- Negative control: Not specified
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