Anti-CD3 antibody (5 µg/mL) or combined with 50 ng/mL PMA (phorbol 12-myristate 13-acetate), 1 µg/mL ionomycin and 2 µM monensin were used to stimulate and activate the cultured splenocytes from the mice.
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Splenocyte Activation Assay
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Corresponding Organization : Hebei University of Chinese Medicine
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Variable analysis
independent variables
- Anti-CD3 antibody (5 µg/mL)
- Anti-CD3 antibody (5 µg/mL) combined with 50 ng/mL PMA (phorbol 12-myristate 13-acetate), 1 µg/mL ionomycin and 2 µM monensin
- Activation of the cultured splenocytes from the mice
- Cultured splenocytes from the mice
- Positive control: Anti-CD3 antibody (5 µg/mL)
- Negative control: Not mentioned
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