Fingerprinting SHTL Powder Compounds

Based on previous reports, 10 different batches of SHTL powders were separated using a ZORBAX SB-C18 column (4.6 × 250 mm, 5 μm, Agilent, Santa Clara, CA, USA) and analyzed for chemical fingerprints using high-performance liquid chromatography (HPLC, Shimadzu Corp., Nakagyo-ku, Kyoto, Japan) coupled with a diode array detector (DAD) (Shimadzu Corp.) [21 (link)]. Acetonitrile in water (solvent A) and 0.4% phosphoric acid in water (solvent B) constituted the mobile phase (Supplementary Table 1). The flow rate was 1.0 mL/min at 25°C, and the detection wavelength was set at 203 nm. Salidroside, chlorogenic acid, paeoniflorin, ferulic acid, luteoloside, ginsenoside Rg1, and luteolin were used as controls to analyze the retention time (Shanghai Yuanye Biotechnology Co., Ltd.; Shanghai, China).