Molecular Profiling of GIST Subtypes

GIST48 and GIST882 cells were established in the Fletcher laboratory (DFCI). All other cells were obtained from ATCC. Etv1^−/− mice, with targeted deletion of the ETS domain, was obtained from the Jessell laboratory (Columbia) and CB17-SCID mice was from Taconic. Antibody sources are: ETV1, ANO1, PGP9.5 (Abcam), KIT for WB, P-Tyr703-KIT (Cell Signaling), P-Tyr204-ERK, GAPDH (Santa Cruz), and anti-mouse Kit for IF (clone ACK2, E-Biosciences).

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Chi P., Chen Y., Zhang L., Guo X., Wongvipat J., Shamu T., Fletcher J.A., Dewell S., Maki R.G., Zheng D., Antonescu C.R., Allis C.D, & Sawyers C.L. (2010). ETV1 is a lineage-specific survival factor in GIST and cooperates with KIT in oncogenesis. Nature, 467(7317), 849-853.

Publication 2010

Ano1 Antibody Cells Clone Deletion Ets domain Gapdh Mouse Pgp9 5 Scid mice

Corresponding Organization : Howard Hughes Medical Institute

Other organizations : Albert Einstein College of Medicine, Brigham and Women's Hospital

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Variable analysis

independent variables

GIST48 cell line
GIST882 cell line
Etv1−/− mice with targeted deletion of the ETS domain
CB17-SCID mice

dependent variables

Measured outcomes not explicitly mentioned

control variables

All other cells obtained from ATCC

controls

Positive control: None specified
Negative control: None specified

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