Synthesized Modular Peptide Dialysis and Characterization

All MDPs were synthesized
on a low loading Rink Amide MBHA resin using an automated synthesizer
with protocols previously reported by our lab.^{25 (link),32 (link)} The crude peptides were dissolved in Milli-Q water and the resulting
solutions were dialyzed. K₂(SL)₆K₂ and K₂(TL)₆K₂ were dialyzed using
100–500 Da cutoff bags, K₂(SL)₆K₂GRGDS and K₂(TL)₆K₂GRGDS using 1000 Da cutoff bags and K(SL)₃RG(SL)₃KGRGDS and K(TL)₂SLRG(TL)₃KGRGDS using 2000 Da cutoff bags. Each peptide solution
was dialyzed against Milli-Q water for 3 days, during which the dialysis
water was refreshed twice daily. Postdialysis the peptide was lyophilized
yielding a white peptide powder. MALDI-TOF mass spectrometry was performed
on a Bruker Autoflex II spectrometer to characterize the purified
peptides.

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Kang M.K., Colombo J.S., D’Souza R.N, & Hartgerink J.D. (2014). Sequence Effects of Self-Assembling MultiDomain Peptide Hydrogels on Encapsulated SHED Cells. Biomacromolecules, 15(6), 2004-2011.

Publication 2014

Autoflex II spectrometer

Maldi Mass spectrometry Peptide Powder Rink amide resin

Corresponding Organization :

Other organizations : Rice University, University of Utah

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Variable analysis

independent variables

Synthesis protocol on a low loading Rink Amide MBHA resin using an automated synthesizer

dependent variables

Characterization of the purified peptides using MALDI-TOF mass spectrometry

control variables

Dialysis of the crude peptides against Milli-Q water for 3 days, with the dialysis water refreshed twice daily
Lyophilization of the peptide solutions to obtain white peptide powder

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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