Western Blot Analysis of Iron Regulators

Western blot analysis was performed as previously described [24 (link)]. Antibodies in this work were anti-KLF14 (ab85476; Abcam, Cambridge, MA, USA), anti-IRP1 (12406–1-AP, Proteintech, 1:1000), anti-IRP2 (23829–1-AP, Proteintech, 1:1000), anti-TfR1 (A5865, ABclonal, 1:1000), anti-Ferritin H (A19544, ABclonal, 1:1000), anti-SIRT1 (A17307, ABclonal, 1:1000), anti-GAPDH (M20006, Abmart, 1:5000), anti-Flag (M20008, Abmart, 1:5000, 1:1000), anti-HA (ab1424，abcam, 1:1000), anti-Actin (ab8227, abcam, 1:1000).

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Zhou H., Chen J., Fan M., Cai H., Dong Y., Qiu Y., Zhuang Q., Lei Z., Li M., Ding X., Yan P., Lin A., Zheng S, & Yan Q. (2023). KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2. Journal of Experimental & Clinical Cancer Research : CR, 42, 5.

Publication 2023

ab85476 A5865 A19544 M20006 M20008 ab1424 ab8227

Actin Antibodies Ferritin Gapdh Irp1 Sirt1 Tfr1 Western blot analysis

Corresponding Organization :

Other organizations : Zhejiang University, First Affiliated Hospital Zhejiang University

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Variable analysis

independent variables

Anti-KLF14
Anti-IRP1
Anti-IRP2
Anti-TfR1
Anti-Ferritin H
Anti-SIRT1
Anti-GAPDH
Anti-Flag
Anti-HA
Anti-Actin

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

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