In the current study, DNA methylation profiling (Illumina Human Methylation 450K Bead Chip Array) and the following clinical data were downloaded from TCGA database. A total of 437 LUAD and 29 control specimens were enrolled in the methylation dataset, and clinical data included the survival time, status, gender, age, and clinical stage. To improve data accuracy, we preprocessed both of the two datasets. If the methylation level of a gene site is NA, which is more than 70% of the total samples, the locus is removed.21 (link) The clinical information of methylation data are shown in Table 1 . The technical route to select the DNA methylated sites signature is shown in Fig. 1 . We download the GSE56044 (n=97) dataset of GEO database for signature independent validation.
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DNA Methylation Profiling in Lung Adenocarcinoma
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Chip
Dna signature
Gene
Human
Corresponding Organization :
Other organizations : Gansu University of Traditional Chinese Medicine, Yangzhou University
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Variable analysis
independent variables
- None explicitly mentioned
- DNA methylation profiling (Illumina Human Methylation 450K Bead Chip Array)
- Survival time
- Status
- Gender
- Clinical stage
- 29 control specimens in the methylation dataset
- Positive control: None mentioned
- Negative control: 29 control specimens in the methylation dataset
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