Western Blot Analyses of Cell Markers

Western blot analyses were performed as previously described^{65 (link),66 (link)}. Primary antibodies were listed as follows: anti-VDAC2 (ab47104, Abcam); anti-PFKP (ab204131, Abcam); anti-β-actin (mAb#5125, Cell Signaling Technology, Danvers, MA); anti-COX IV (#4850, Cell Signaling Technology); anti-OLIG2 (ab109186, Abcam, Cambridge, UK); anti-β-tubulin (ab179513, Abcam); anti-CD133 (Miltenyi Biotec, Bergisch Gladbach, Germany); and anti-SOX2 (#3579, Cell Signaling Technology). Secondary antibodies are horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H + L) (A0208, Beyotime, Shanghai, China) and HRP-labeled goat anti-mouse IgG (H + L) (A0216, Beyotime). Immuno-bands were quantified by densitometry using ImageJ software (NIH, Bethesda, MD, USA).