IHC Staining of TELO2 in Bladder Cancer

IHC staining of commercially available tissue microarray (BS17016; Biomax, Rochester, NY, USA) was performed according to previous protocol [29 (link)–31 (link)], incubated with a polyclonal rabbit anti-human TELO2 antibody (Cat. No. ab122722, Abcam, Cambridge, UK) (1:100 diluted in phosphate buffered saline (PBS) for 1 h at room temperature, washed 3 times (each for 5 min in PBS), incubated with biotin-labeled secondary immunoglobulin (1:100, DAKO, Glostrup, Denmark) for 1 h at room temperature, washed 3 times, and treated with 3-amino-9-ethylcarbazole substrate chromogen (DAKO) at room temperature to visualize peroxidase activity [32 (link)]. Sections of human urinary bladder cancer tissue (known to stain positive for TELO2) were used as positive control according to the datasheet of TELO2 antibody (ab122722).