Western Blot Analysis of Protein Targets

Western blot analysis was performed, as described previously.24 (link) Anti-mouse phosphorylated STAT5 (pSTAT5), pSTAT6, pIKKα/β, Bax, Bcl2, Caspase3, cleaved Caspase3, Bim, p50, p65, and β-actin were purchased from Cell Signaling Technology (CST).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Yang S., Chen J., Zhang H., Jiang Y., Qin T., Gao S., Yue Y, & Wang S. (2020). TNF-a Is a Potent Stimulator of Tc9-Cell Differentiation. Journal of Immunotherapy (Hagerstown, Md. : 1997), 43(9), 265-272.

Publication 2020

pIKKα/β Bax Bcl2 Caspase3 cleaved caspase3 β-actin

Actin Bcl2 Caspase3 Mouse Stat5 Western blot analysis

Corresponding Organization :

Other organizations : Wenzhou Medical University, Zhejiang Taizhou Hospital, First Hospital of Jilin University, Jilin University

Top 5 similar protocols

Variable analysis

independent variables

Anti-mouse phosphorylated STAT5 (pSTAT5)
PSTAT6
PIKKα/β
Caspase3
Cleaved caspase3

dependent variables

Western blot analysis

control variables

β-actin

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!