The radioactivity assay, adrenochrome, and fluorogenic assays were performed as described previously,[9 (link),13 (link),25 (link)] using tritium labeled cis-stilbene oxide, epibromohydrin (Sigma), and CMNGC (3 ) as substrates, respectively. For assays with CMNGC, measurements were taken using a fluorescent plate reader with λex = 330 nm and λem = 465 nm.
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Radioactivity, Adrenochrome, and Fluorogenic Assays
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Other organizations : Dartmouth College, University of California, Davis
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Variable analysis
independent variables
- Tritium labeled cis-stilbene oxide
- Epibromohydrin (Sigma)
- CMNGC (3)
- Radioactivity
- Adrenochrome
- Fluorescence (measured using a fluorescent plate reader with λex = 330 nm and λem = 465 nm)
- Measurement procedures for radioactivity assay, adrenochrome assay, and fluorogenic assay as described previously in references [9], [13], and [25], respectively
- Not specified
- Not specified
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