Glucose tolerance test was performed after a 12-hour fast, and blood samples
collected from caudal artery were used for glucose analysis. Intraperitoneal
administration of 20% glucose (glucose monohydrate, Merck, São Paulo, Brazil)
(2 g/kg) was performed, followed by measurement of glucose levels at 30, 60, 90, 120,
180 and 240 minutes7 (link),10 , using a glucose meter (ACCU-CHEK GO
KIT, Roche Diagnostic Brazil Ltda, SP, Brazil).
Two days after in vivo analysis, the animals were kept under fasting
for 12 hours, anaesthetized intraperitoneally with sodium pentobarbital (50 mg/kg)
and euthanized by decapitation. Blood samples were collected, centrifuged (3,000 rpm)
and stored for further analysis. Biochemical analyses included measurements of serum
glucose, triglycerides, total cholesterol, HDL, LDL, albumin and total protein, using
an enzymatic method with specific kits.
collected from caudal artery were used for glucose analysis. Intraperitoneal
administration of 20% glucose (glucose monohydrate, Merck, São Paulo, Brazil)
(2 g/kg) was performed, followed by measurement of glucose levels at 30, 60, 90, 120,
180 and 240 minutes7 (link),10 , using a glucose meter (ACCU-CHEK GO
KIT, Roche Diagnostic Brazil Ltda, SP, Brazil).
Two days after in vivo analysis, the animals were kept under fasting
for 12 hours, anaesthetized intraperitoneally with sodium pentobarbital (50 mg/kg)
and euthanized by decapitation. Blood samples were collected, centrifuged (3,000 rpm)
and stored for further analysis. Biochemical analyses included measurements of serum
glucose, triglycerides, total cholesterol, HDL, LDL, albumin and total protein, using
an enzymatic method with specific kits.
