Transgenic Bnip3 Overexpression in Mice

Transgenic over-expression of Bnip3 in C57BL/6 mice was achieved by standard transgenic procedures using the α-MHC promoter to direct expression selectively to the myocardium. Three founders were backcrossed to wild type C57BL/6 mice for 3 generations to obtain stable lines. Cardiac functions were monitored using a Visual Sonics Vevo-770 imaging system (Toronto, Canada). Curcumin (100 mg/kg) or vehicle (1% gum arabic) was administered to animals once a day by gavage. Hearts were sectioned and stained as previously described [18 (link)].

Free full text: Click here

Thompson J.W., Wei J., Appau K., Wang H., Yu H., Spiga M.G., Graham R.M, & Webster K.A. (2015). Bnip3 Binds and Activates p300: Possible Role in Cardiac Transcription and Myocyte Morphology. PLoS ONE, 10(8), e0136847.

Publication 2015

Vevo-770 imaging system

Animals C57bl mice Curcumin Gavage Gum arabic Hearts Myocardium Transgenic

Corresponding Organization :

Other organizations : University of Miami, Second Affiliated Hospital of Zhejiang University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Transgenic over-expression of Bnip3 in C57BL/6 mice
Curcumin (100 mg/kg) or vehicle (1% gum arabic) administration

dependent variables

Cardiac functions monitored using a Visual Sonics Vevo-770 imaging system
Heart histological staining and analysis

control variables

C57BL/6 mouse strain
Three generations of backcrossing to wild type C57BL/6 mice to obtain stable lines

controls

Positive control: Transgenic over-expression of Bnip3 in C57BL/6 mice
Negative control: Vehicle (1% gum arabic) administration

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!