Transmission Electron Microscopy of Microbial Cells

The results of the SEM were confirmed by TEM analysis, as discussed elsewhere [10 (link)]. Briefly, MRSA, MDR-PA, and C. albicans cells grown in the presence of MIC values of TCE-Ag-3 were centrifuged, washed with MQ water, fixed in 2.5% glutaraldehyde and 1% osmium tetraoxide, and embedded in Epon resin (Polybed 812). So prepared blocks of cells were sectioned, stained with uranyl acetate and lead citrate (Sigma Aldrich, St. Louis, MO), and imaged on TEM (Morgagni 268, FEI, Czech Republic) with accelerating voltage of 80 kV.

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Ansari M.A., Kalam A., Al-Sehemi A.G., Alomary M.N., AlYahya S., Aziz M.K., Srivastava S., Alghamdi S., Akhtar S., Almalki H.D., Adil S.F., Khan M, & Hatshan M.R. (2021). Counteraction of Biofilm Formation and Antimicrobial Potential of Terminalia catappa Functionalized Silver Nanoparticles against Candida albicans and Multidrug-Resistant Gram-Negative and Gram-Positive Bacteria. Antibiotics, 10(6), 725.

Publication 2021

uranyl acetate lead citrate Morgagni 268

C albicans Cells Citrate Epon Glutaraldehyde Mrsa Osmium tetraoxide Polybed 812 Resin Uranyl acetate

Corresponding Organization : King Saud University

Other organizations : Imam Abdulrahman Bin Faisal University, King Abdulaziz City for Science and Technology, University of Allahabad, Umm al-Qura University

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Variable analysis

independent variables

MIC values of TCE-Ag-3

dependent variables

Morphology and ultrastructure of MRSA, MDR-PA, and C. albicans cells

control variables

Centrifugation of cells
Washing with MQ water
Fixation in 2.5% glutaraldehyde and 1% osmium tetraoxide
Embedding in Epon resin (Polybed 812)
Sectioning of cell blocks
Staining with uranyl acetate and lead citrate
Imaging on TEM (Morgagni 268, FEI, Czech Republic) with accelerating voltage of 80 kV

controls

Positive control: Not specified
Negative control: Not specified

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