Histological Analysis of Ovarian Cancer

Tumors excised from the peritoneal cavity of 32 patients with epithelial ovarian cancer (stage III and IV) as well as fragments of tumor-free peritoneum from the same patients were included in the study. The age of patients ranged from 42 to 60 years. The tissues were fixed in 4% formalin and were then processed as described in^{11 (link)}. The cancer tissue was identified using standard H + E staining. Peritoneal mesothelium located near the cancer cells was identified based on positive stainings of the D2-40, Wt-1, HBME-1, and calretinin with specific antibodies (Dako, Santa Clara, CA). Antigen visualization was performed using an Envision Flex (Dako).

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Pakuła M., Witucka A., Uruski P., Radziemski A., Moszyński R., Szpurek D., Maksin K., Woźniak A., Sajdak S., Tykarski A., Mikuła-Pietrasik J, & Książek K. (2019). Senescence-related deterioration of intercellular junctions in the peritoneal mesothelium promotes the transmesothelial invasion of ovarian cancer cells. Scientific Reports, 9, 7587.

Publication 2019

D2-40 HBME-1 calretinin specific antibodies Envision Flex

Antibodies Antigen Calretinin Cancer Cells Epithelial ovarian cancer Formalin Mesothelium Patients Peritoneal Peritoneal cavity Peritoneum tumor Stainings Tissue Tumors

Corresponding Organization :

Other organizations : Poznan University of Medical Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Identification of cancer tissue using standard H + E staining
Identification of peritoneal mesothelium located near the cancer cells based on positive stainings of the D2-40, Wt-1, HBME-1, and calretinin with specific antibodies

control variables

Age of patients ranged from 42 to 60 years
Tissues were fixed in 4% formalin and then processed as described in the referenced study

controls

Positive control: Peritoneal mesothelium located near the cancer cells identified based on positive stainings of the D2-40, Wt-1, HBME-1, and calretinin with specific antibodies
Negative control: Not explicitly mentioned

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