With slight modifications according to the previous method [38 (link)], oocytes were washed in 0.1% PVA-PBS, incubated with 10 μg/mL Nile red solution (Solarbio, Beijing, China) for 10 min, and observed under the epifluorescence inverted microscope (Nikon, Tokyo, Japan) connected to a DSRi1 camera (Nikon, Tokyo, Japan). Fluorescent images were analyzed by the EZ-C1 Free Viewer software (Nikon, Tokyo, Japan).
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