Immunohistochemical analysis of brain tissue
Corresponding Organization :
Other organizations : Geriatric Research Education and Clinical Center, University of Puget Sound, University of Washington, Uniformed Services University of the Health Sciences, Center for Neuroscience and Regenerative Medicine
Variable analysis
- Blast-treated animals
- Sham-treated animals
- EAAT4 expression
- Calbindin expression
- GLUT1 expression
- ICAM-1 expression
- Tissue fixation in 4% paraformaldehyde in PBS at 4°C for ~60 h
- Tissue equilibration in 30% sucrose in PBS for 24 h at 4°C
- Tissue embedding in OCT
- Antigen retrieval with 50 mM sodium citrate (pH 9.0) and heating at 80°C for 30 min
- Immunostaining protocol as previously described
- Confocal microscopy with Leica TCS SP5 II microscope
- Image acquisition with Leica Application Suite and processing with linear contrast and brightness adjustments applied equally to blast- and sham-treated animals
Annotations
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