Comparative Analysis of MMP1 Expression in ESCC

To compare the expression of MMP1 in other studies, we retrieved two publicly available sets of microarray data (GSE2340024 (link) and GSE2034725 (link)) from the Gene Expression Omnibus (GEO) database. GSE23400 and GSE 20347 consist of gene expression data from 53 and 17 Chinese ESCC patients, respectively. The MMP1 levels in tumors and corresponding normal specimens were compared and T/N ratios were calculated.
Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Free full text: Click here

Chen Y.K., Tung C.W., Lee J.Y., Hung Y.C., Lee C.H., Chou S.H., Lin H.S., Wu M.T, & Wu I.C. (2016). Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma. Scientific Reports, 6, 30057.

Publication 2016

ChemMate DAKO EnVision Detection Kit

Anti antibody Chinese Dilution Embedded paraffin Formalin Gene expression Immunohistochemistry Microarray Mmp1 Mouse Patients Rabbit Tumors

Corresponding Organization :

Other organizations : Meiho University, Kaohsiung Medical University, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung Municipal Hsiao-Kang Hospital, National Yang Ming Chiao Tung University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

MMP1 expression levels in tumors and corresponding normal specimens

control variables

None explicitly mentioned

controls

Positive control, Not specified
Negative control, Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!