Quantitative Analysis of SCFAs

SCFAs were extracted from cecal content (~2 g) and analyzed by using gas-liquid chromatography (GLC) as described previously [34 (link),35 (link)]. Briefly, the cecal content was homogenized and protonated with hydrochloric acid, by using 2-ethylbutyric acid as internal standard. Samples were centrifuged before injection onto a fused-silica capillary column (DB-FFAP 125-3237; J&W Scientific, Agilent Technologies Inc., Folsom, CA, USA). SCFAs in serum were pre-enriched and extracted by hollow-fiber before being injected and analyzed with GLC using the same column as for SCFAs extracted from cecum. GC ChemStation software (Agilent Technologies Inc.,Wilmington, DE, USA) was used for evaluation of the SCFA analysis. For analysis of lactic and succinic acids ion-chromatography was employed according to Jakobsdottir et al. [10 (link)]. The same extraction as for SCFAs was used.

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Liu H.Y., Walden T.B., Cai D., Ahl D., Bertilsson S., Phillipson M., Nyman M, & Holm L. (2019). Dietary Fiber in Bilberry Ameliorates Pre-Obesity Events in Rats by Regulating Lipid Depot, Cecal Short-Chain Fatty Acid Formation and Microbiota Composition. Nutrients, 11(6), 1350.

Publication 2019

DB-FFAP 125-3237 GC ChemStation software

Acid Capillary Cecum Chromatography Fiber Gas liquid chromatography Hydrochloric acid Serum Silica Succinic acids

Corresponding Organization : University of California, Davis

Other organizations : Science for Life Laboratory, Lund University

Top 5 similar protocols

Variable analysis

independent variables

Extraction of SCFAs from cecal content

dependent variables

SCFA concentrations in cecal content analyzed by gas-liquid chromatography (GLC)
Lactic and succinic acid concentrations in cecal content analyzed by ion-chromatography

control variables

Use of 2-ethylbutyric acid as internal standard for SCFA analysis
Use of the same GLC column (DB-FFAP 125-3237) for analyzing SCFAs in both cecal content and serum

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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