Paraffin-embedded mouse and human kidney sections (2.0-μm-thick) were prepared as described previously 25 (link). A routine Hematoxylin-eosin (H&E) staining of the mouse kidney section was performed in accordance with protocol. DUSP2 expression was quantified using Image Pro-Plus v. 6.0 software (Bethesda, MD, USA).
Immunohistochemical (IHC) staining was performed as described previously 26 (link). Briefly, after de-waxing, hydration, endogenous enzyme, and biotin removal and antigen repair, the sections were incubated with primary antibodies at 4 °C overnight. Then, the Cy3 (Goat Anti-Mouse IgG, Abcam; Goat anti-Rabbit IgG, Invitrogen)- or Alexa Fluor 488-conjugated-conjugated secondary antibody (Goat Anti-Mouse IgG, Abcam; Goat anti-Rabbit IgG, Invitrogen) were incubated for 1 h at room temperature before DAPI (Beyotime Biotechnology, Shanghai, China) staining. PBS was used as a negative control instead of a primary antibody. Slides were photographed using a confocal microscope (ZEISS, LSM780) and analyzed with ZEN 2012 software (version 1.1.1.0, Carl Zeiss Microscopy GmbH).
Immunohistochemical (IHC) staining was performed as described previously 26 (link). Briefly, after de-waxing, hydration, endogenous enzyme, and biotin removal and antigen repair, the sections were incubated with primary antibodies at 4 °C overnight. Then, the Cy3 (Goat Anti-Mouse IgG, Abcam; Goat anti-Rabbit IgG, Invitrogen)- or Alexa Fluor 488-conjugated-conjugated secondary antibody (Goat Anti-Mouse IgG, Abcam; Goat anti-Rabbit IgG, Invitrogen) were incubated for 1 h at room temperature before DAPI (Beyotime Biotechnology, Shanghai, China) staining. PBS was used as a negative control instead of a primary antibody. Slides were photographed using a confocal microscope (ZEISS, LSM780) and analyzed with ZEN 2012 software (version 1.1.1.0, Carl Zeiss Microscopy GmbH).
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