Fecal SCFA Profiling by LC-MS/MS

Fecal samples were collected on dry-ice and stored at 80 °C until analysis. Concentrations of eight short-chain fatty acids: acetic acid (C2, acetate), propionic acid (C3, propionate), isobutyric acid (C4), butyric acid (C4, butyrate), 2-methyl-butyric acid (C5), isovaleric acid (C5), valeric acid (C5) and caproic acid (hexanoic acid, C6) were assessed by LC–MS/MS (Metabolon, Morrisville, NC), using their Metabolon Method TAM135: “LC–MS/MS Method for the Quantitation of Short Chain Fatty Acid (C2 to C6) in Human Feces” workflow.

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Amato-Menker C.J., Hopen Q., Pettit A., Gandhi J., Hu G., Schafer R, & Franko J. (2024). XX sex chromosome complement modulates immune responses to heat-killed Streptococcus pneumoniae immunization in a microbiome-dependent manner. Biology of Sex Differences, 15, 21.

Publication 2024

LC–MS/MS

Corresponding Organization :

Other organizations : West Virginia University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Concentrations of eight short-chain fatty acids: acetic acid (C2, acetate), propionic acid (C3, propionate), isobutyric acid (C4), butyric acid (C4, butyrate), 2-methyl-butyric acid (C5), isovaleric acid (C5), valeric acid (C5) and caproic acid (hexanoic acid, C6)

control variables

Fecal samples were collected on dry-ice and stored at 80 °C until analysis

controls

No positive or negative controls were explicitly mentioned in the provided information.

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