ROS generation in cells was measured using ESR, as previously described (19 (link)). Briefly, 106 cells were seeded on a glass cover slide (49.0×5.0×0.2 mm) and incubated overnight as previously described. Cells were treated with 0 or 1,000 µM TP4O for 15 min. ESR was measured in a respiration buffer containing 10 mM DMPO as the spin-trapping agent. All ESR spectra were obtained using a JES-RE1X X-band spectrometer (JEOL, Ltd., Tokyo, Japan).
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ROS Generation Measurement via ESR
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Other organizations : University of Tsukuba
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Variable analysis
independent variables
- TP4O concentration (0 or 1,000 µM)
- ROS generation in cells (measured using ESR)
- Cell density (10^6 cells)
- Incubation time (overnight)
- Spin-trapping agent (10 mM DMPO)
- ESR spectrometer (JES-RE1X X-band spectrometer)
- Negative control: 0 µM TP4O
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