Immunohistochemical Profiling of Cell Signaling

Immunohistochemistry (IHC) for all targets was performed using standard protocols on the automated immunohistochemistry staining system Discovery XT (Roche/Ventana, Tucson, Arizona, USA) and the LEICA BOND-III automated stainer (Leica, Wetzlar, Germany) respectively. The following antibodies were used: PD-L1 (Cell Signaling, Boston, U.S.A.), p-S6K1 (Cell Signaling, Boston, U.S.A.), p-4EBP1 (Cell Signaling, Boston, U.S.A.), p-RPS6 (Ser 235/236 and Ser240/244, Cell Signaling, Boston, U.S.A.), p-PRAS40 (Cell Signaling, Boston, U.S.A.), p-NDRG1 (Cell Signaling, Boston, U.S.A.), p-mTOR (S2448, Cell Signaling, Boston, U.S.A.), IDH1_R132H (Dianova, Eching, Germany) and BRAF V600E (DCS, Hamburg, Germany) [27 (link)].

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Luger A.L., König S., Samp P.F., Urban H., Divé I., Burger M.C., Voss M., Franz K., Fokas E., Filipski K., Demes M.C., Stenzinger A., Sahm F., Reuss D.E., Harter P.N., Wagner S., Hattingen E., Wichert J., Lapa C., Fröhling S., Steinbach J.P, & Ronellenfitsch M.W. (2022). Molecular matched targeted therapies for primary brain tumors—a single center retrospective analysis. Journal of Neuro-Oncology, 159(2), 243-259.

Publication 2022

Discovery XT LEICA BOND-III PD-L1 p-S6K1 p-4EBP1 p-RPS6 p-PRAS40 p-NDRG1 p-mTOR IDH1_R132H

4ebp1 Antibodies Braf Mtor Ndrg1 Pd l1

Corresponding Organization : University Hospital Frankfurt

Other organizations : Frankfurt Cancer Institute, University Hospital Heidelberg, German Cancer Research Center, University of Augsburg

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Variable analysis

independent variables

Antibodies used for immunohistochemistry (IHC) staining: PD-L1, p-S6K1, p-4EBP1, p-RPS6 (Ser 235/236 and Ser240/244), p-PRAS40, p-NDRG1, p-mTOR (S2448), IDH1_R132H, and BRAF V600E

dependent variables

Immunohistochemical staining patterns and intensities of the target proteins in the samples

control variables

Standard IHC protocols
Automated immunohistochemistry staining systems: Discovery XT (Roche/Ventana) and LEICA BOND-III

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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