Propagation and Maintenance of Cell Lines

NHDFs (Lonza, CC-2509) were cultured in Dulbecco's modified Eagle's medium (DMEM; Corning, 10-013-CV) supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, and 5% (v/v) fetal bovine serum (FBS; Invitrogen). Vero cells (American Type Culture Collection) were propagated in DMEM supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, and 5% (v/v) calf serum (FBS; Invitrogen). BSC40 cells were maintained in DMEM supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, and 10% (v/v) FBS. HCMV AD169GFP was kindly provided by Dong Yu and was propagated in NHDFs as described (Bianco and Mohr 2017 (link)). Vaccinia virus (Western Reserve strain) was propagated in BSC40 cells as described (Burgess and Mohr 2015 (link)). Wild-type HSV-1 (Patton strain) expressing an EGFP-Us11 fusion protein (Benboudjema et al. 2003 (link)) was propagated in Vero cells. PP242 (P0037) was purchased from Sigma and used at a concentration of 25 µM.