Beckman Coulter’s DxI800 Immunoassay System was using to detecting Serum p2PSA, tPSA and fPSA of each patient. PHI was calculated by ([p2PSA/fPSA]×. Performance of individual PSA and PHI measurements in discriminating clinical outcomes was measured using the detection rate of PCa, sensitivity, specificity, and the area under the receiver operating characteristic curve (AUC) (1 (link),6 (link)).