In this study, two LC-MS/MS acquisition methods were used to determine the toxin profile of the strains of G. polynesiensis: method 1 for the P-CTX group based on the protocol by Sibat et al., 2018 [51 (link)] and method 2 for MTXs and gambierone group toxins [29 (link)]. A brief description is given below.
Both experiments were performed using a UHPLC system (UFLC Nexera, SHIMADZU, Kyoto, Japan) coupled to a hybrid triple quadrupole-linear ion-trap API4000 QTRAP mass spectrometer (SCIEX, Redwood city, CA, USA) equipped with a TurboV® electrospray ionization source (ESI). The instrument control, data processing, and analysis were conducted using Analyst software 1.6.2 (Sciex, CA, USA).
Both experiments were performed using a UHPLC system (UFLC Nexera, SHIMADZU, Kyoto, Japan) coupled to a hybrid triple quadrupole-linear ion-trap API4000 QTRAP mass spectrometer (SCIEX, Redwood city, CA, USA) equipped with a TurboV® electrospray ionization source (ESI). The instrument control, data processing, and analysis were conducted using Analyst software 1.6.2 (Sciex, CA, USA).
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